Kick starting 2025 with Our First Webinar organized by WG1!
Title: Transient Naive Treatment (TNT) Reprogramming iPSC
Date: 20 February 2025
Time: 10:00 CET
Location: Zoom (Zoom link will be sent the day before the event))
The Transient Naive Treatment (TNT) reprogramming method, which briefly introduces naive media early in the reprogramming process, effectively erases epigenetic memory and produces induced pluripotent stem (iPS) cells more similar to human embryonic stem (hES) cells than conventional reprogramming. TNT reprogramming was shown to mitigate reprogramming-induced epigenetic aberrations, particularly in regions marked by H3K9me3, lamin-B1, and aberrant CpH methylation, without impacting genomic imprinting. Furthermore, Sendai virus expression is absent in TNT iPSCs, reinforcing its potential as a standard for biomedical applications.
Presenters:
Sam Buckberry, Australian National University, Canberra, Australia, and The Kids Research Institute, Perth, Australia
Xiaodong Liu, Westlake University, Hangzhou, China and Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China
Daniel Poppe, University of Western Australia, Perth, Australia and Harry Perkins Institute of Medical Research, Perth, Australia
Jia Ping Tan, Westlake University, Hangzhou, China and Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China
Link to the publications:
Buckberry S, Liu X, Poppe D, Tan JP, Sun G, Chen J, Nguyen TV, de Mendoza A, Pflueger J, Frazer T, Vargas-Landín DB, Paynter JM, Smits N, Liu N, Ouyang JF, Rossello FJ, Chy HS, Rackham OJL, Laslett AL, Breen J, Faulkner GJ, Nefzger CM, Polo JM, Lister R. Transient naive reprogramming corrects hiPS cells functionally and epigenetically. Nature. 2023 Aug;620(7975):863-872.
https://www.nature.com/articles/s41586-023-06424-7
Sam Buckberry, Xiaodong Liu, Daniel Poppe, Jia Ping Tan, Geoffrey J. Faulkner, Jose M Polo, Ryan Lister. Transient Naive Treatment (TNT) iPS cells do not feature Sendai virus expression: Response to Sendai virus persistence questions the transient naive reprogramming method for iPSC generation. bioRxiv 2024.07.10.602807; doi: https://doi.org/10.1101/2024.07.10.602807
Please note that the webinar is only for registered members of CorEuStem, and the Zoom link will be sent only to members. There is still the possibility to join CorEuStem via https://coreustem.eu/.
